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Improving Heat Detection in Tie-Stall and Free-Stall Environments


Jack H. Britt

College of Veterinary Medicine,
North Carolina State University,
4700 Hillsborough St.,
Raleigh, NC, USA 27606
E-mail: jack_britt@ncsu.edu

Take Home Messages
The surest sign of estrus is standing-to-be-mounted and cows detected by this behavioral sign have the highest fertility.
The most important factors that affect expression of estrus are number of animals in the sexually active group, footing conditions, and interval since calving.
Hormonal regulation of estrus can increase the number of sexually active animals and focus the heat detection effort on a predictable period of time.
Activity monitors combined with computerized identification and recording systems can partially automate detection of heat, but careful evaluation of data is required for accurate detection.

Introduction


Catching cows in heat and breeding them at the right time using the correct artificial insemination procedures are necessary steps for successful reproductive management of a dairy herd. Regrettably, these steps can be neglected for days, weeks or even months before the economic consequences become apparent. Thus, the greatest challenge that one faces in motivating personnel to do a better job in these areas is persuading them that poor habits in catching heats and breeding cows lead to higher costs and lower incomes. The net daily economic difference due to "reproductive costs" between a cow that is open 12 months and one that is open 15 is equivalent to the difference between a cow producing 23 kg per day and one producing 34. This presentation will focus on factors that influence detection of estrus in dairy cattle, and how an understanding of these factors may help one improve reproductive management practices in herds housed in different systems. More comprehensive reviews of this subject have been presented recently (2, 8, 20).

What Causes Cows to Show Heat?


The hormonal condition that can lead to expression of heat in the cow is a high blood level of estrogen (estradiol-17) in the presence of a low level of progesterone (25). The absolute amount of estrogen is not critical as long as it is above a threshold (approximately 10 pg/ml). The amount of progesterone is critical and it needs to be below a relatively low threshold (about 0.6 ng/ml). This hormonal situation normally exists when there is a mature pre-ovulatory follicle secreting estrogen in the absence of a functional corpus luteum. Other conditions that can lead to expression of heat include presence of follicular cysts and occasional unpredictable conditions in pregnant cows (23).

Although high estrogen and low progesterone are considered the hormonal prerequisites for expression of heat, these hormones are not the ultimate signals that induce signs of heat. The specific ultimate signals are unknown, but they include classical neurotransmitters and possibly neuropeptides. Some physiological signs of heat such as edema and hyperemia of the genitalia and secretion of cervical and vaginal mucus are involuntary. But the more important behavioral signs such as standing, mounting, licking, butting and head-resting are voluntary reflexes that are influenced greatly by existing conditions in the cow's immediate environment (1).

What Affects Expression of Heat?


Involuntary signs of estrus are not influenced by a cow's immediate environment. For example, hyperemia of the genitalia and secretion of mucus are not influenced by weather, footing conditions or other factors that influence behavioral signs. In contrast, voluntary behavioral signs of estrus are subject to many influences (3). The ones that are most important on most dairy farms are:

number of animals in the sexually active group,
space and freedom for sexually active animals to interact,
interfering activities such as feeding,
footing conditions, and
stressors such as high temperatures or negative energy balance.

Behavioral signs of heat generally require that at least two animals interact. Studies in our laboratory indicate that secondary behavioral signs such as butting, licking and head-resting are influenced less by a cow's immediate environmental conditions than are the primary behavioral signs, mounting and standing (24). Most experienced observers utilize these secondary signs to pick out cows that are most likely to be in heat even when the immediate environmental conditions limit mounting and standing activity.

A cow will not be detected to stand if there is no other animal willing to mount. Mounting activity is stimulated strongly by estrogen and inhibited strongly by progesterone (25). Thus, mounting frequency is considerably greater for cows in proestrus or estrus than for cows that are out of heat or in midcycle (9, 25). Once there are four or more sexually active animals (proestrus or estrus) in a group, then mounting activity will normally be sufficient for efficient detection of heat (9). Mounting behavior has a fairly high repeatability, and cows that are active mounters during one lactation are also more active during their next lactation (25).

Mounting activity is influenced considerably by the cow's immediate environment. We studied mounting and standing activity in high producing Holstein cows during six months after calving (4). Ovaries of these cows were removed and then the cows were given low doses of estrogen every four weeks throughout lactation to stimulate sexual behavior. We found that standing activity was constant throughout the six months after calving, and that higher producers tended to stand more than lower producers. In contrast, mounting activity increased during the six months after calving. Mounting activity was also higher in high producing cows. The most important observation that we made was associated with the location where cows were checked for heat. During the study, cows were watched for 1 hr every 8 hr after the estrogen treatment. During each of these 1-hr checks, the cows were on dirt for 30 min and on a dry grooved concrete alley for 30 min. The results are shown in Table 1.

These results show quite clearly that mounting and standing activity and length of estrus were greater on dirt than on concrete. When the length of heat was estimated from observations on dirt, the average heat lasted nearly 14 hours, and 86% of the heats were longer than 12 hr. However, when observations on concrete were used to estimate these same variables, we found that the average heat lasted about 9 hr and only 12% of the cows were in heat for more than 12 hr. Thus the same cows during the same heat periods expressed substantially more activity if they had access to a dirt lot. This has important implications in designing a heat detection program. It suggests that getting cows off concrete when they are checked for heat should result in better efficiency in detection of heat. If cows are moved onto dirt lots for heat detection, it is advisable to have a well-drained lot so footing conditions are good.

We also found that mounting activity was greatest just after cows were moved to a different location, particularly if this location was a dirt lot with good footing (4, 24). We have noted that cows reduce physical activity such as mounting when the footing surface is poor, but they do not reduce secondary sexual behaviors such as licking the vulva, chin resting, butting and other agonistic behaviors seen in cows that are in heat.

Table 2 indicates relative mounting activity that one might expect to observe in various locations and conditions on dairy farms. These empirical values are based on data from several published and unpublished studies and on casual observations made on many farms. A value of 1.0 is assigned to mounting activity expected to occur on a relatively dry grooved concrete alley. A higher index means more mounting activity.

Activities or conditions that restrict interactions among cows influence whether cows show heat; therefore, heat checks should be scheduled to occur when cows are free to interact and not after they have been fed or when they are being moved or are crowded (1). Cows that are eating, crowded in holding pens or alleys do less mounting. Cows that are on slippery alleys, frozen ground or any surface that makes footing tenuous show less mounting activity. One study from our laboratory revealed that cows in heat are more likely to mount another cow in heat if the other cow is loose rather than tied (25). Perhaps this indicates that freedom to interact before mounting is important for maximum expression of mounting activity. Cows that have foot problems, regardless of whether the problem is structural, subclinical or clinical, apparently show poorer reproductive performance (5), and this may be related in part to poorer expression of estrus. Many of the foot problems that affect mounting activity can be alleviated by proper foot care (foot baths and trimming) and getting cows off concrete surfaces for detection of estrus.

There is no firm experimental evidence that high levels of milk production per se influence mounting or standing activity. There is evidence that energy balance during the early postpartum period may influence whether a cow is detected in heat at the beginning of the first or second postpartum cycle (7, 21). Apparently cows experiencing a severe negative energy balance can produce enough estrogen to elicit an LH surge, but the low energy balance interferes with behavioral expression of heat. Similarly, there is evidence that cows treated with bovine somatotropin may have poorer expression of estrus than non-treated herdmates (13), probably because of differences in energy balance.

Certain kinds of stress may also suppress estrus or shorten its duration. Intact or ovariectomized estrogen-treated cows and heifers showed reduced expression of estrus when challenged with some hormones of the anterior pituitary-adrenal axis (2). Thus, either corticotropin or synthetic glucocorticoids (but not natural corticoids) reduced duration of estrus and altered the interval from induced luteolysis to estrus. These data provide circumstantial evidence that cattle subjected to stressful conditions that would activate the hypothalamic-pituitary- adrenal axis might show poorer signs of estrus.

Extremes in temperature affect intensity of heat. Mounting activity is lower on days when the ambient temperature is greater than or substantially lower than the cow's thermoneutral zone. Heats may appear to be shorter when the temperatures are extreme, but it isn't clear whether this is because of less mounting activity or because of less willingness to stand.

Detection of Heat in Dairy Herds


Interval from calving until when cows are first detected in heat depends on when cows begin to cycle and on the intensity of heat at the first three to five cycles. Well-fed, healthy cows should begin cycling by the second to fourth week postpartum (Table 3). Intensity of estrus is lower at the first cycle postpartum than at the second and third cycles (1, 2), and this is reflected in an increase in estrous detection percentages in practice (Table 4).

The duration of estrus ranges from about 3 to 24 hours with an average of about 12 to 15 hours for milking cows. Thus heats can be missed easily if observations are not scheduled properly and if conditions are not favorable for maximum expression of heat. The most important behavioral sign of heat requires that at least two animals interact, because a cow will not be detected to stand if there is no other animal willing to mount. Conception rates are higher for cows inseminated on the basis of standing heat than on the basis of secondary signs (19). Part of this may be due to fact that cows that are in heat for more than 12 hours (two twice-daily heat checks) apparently have higher fertility than those in heat at only one check. But a great deal of the differences among herds in conception rates is related to accuracy of detection and timing of insemination. Cows inseminated on the basis of standing-to-be-mounted are more likely to be in heat and are more likely to be bred at the correct time than those bred on the basis of other signs.

Heat detection accuracy is a major problem on many farms. On the average farm, about 10 to 20% of cows submitted for insemination have high levels of progesterone and therefore are not likely to be in heat (15). Even cows with low progesterone on the day of submission may not be in heat, because the period of low progesterone encompasses about five to seven days of a cycle. Milk progesterone testing can be a useful tool for improving accuracy of heat detection. Some pregnant cows with high levels of progesterone will show estrus (23). Estrus during pregnancy occurs more often after the fourth month of pregnancy, occurs more often in fourth or fifth parities, occurs when progesterone is high and estradiol generally low, and is of shorter duration (5.6 hr) than is typical for non-pregnant estrual cows. Pregnant cows that show estrus are part of the sexually active group, and some cows have been reported to show estrus repeatedly during consecutive pregnancies (23).

Heat Detection Aids


Heat detection aids can influence detection rate and accuracy, depending on how they are used and how the data are interpreted (20). The most-widely used heat detection aids are the hormones (prostaglandins, progestogens and estrogens) that are used to synchronize estrus in groups of animals or induce estrus in individual anestrous animals. Other heat detection aids comprise those that detect increased physical activity such as walking or standing-to-be-mounted (20), those that detect physiological changes such as electrical impedance of the vaginal submucosa or changes in hormone concentrations in milk (15, 20), and those that utilize hormone-treated detector animals with increased sexual behavior (10, 14).

Hormonal-Controlled Estrus

One of the main opportunities for use of estrous synchronizing hormones in dairy herds is for predictable induction of estrus in batches of cows or heifers for enhancement of efficiency of detection of estrus. The popularity of using such programs has increased in recent years, partly because of larger-sized herds and partly because of new systems for using the hormonal treatments. Typical programs include those in which eligible cows are set up by injection of a luteolytic prostaglandin about 17 days before the end of the voluntary waiting period and then these cows are given a second prostaglandin injection three days before breeding is to begin. Such treatments allow producers to focus heat-detection efforts on groups of cows at designated times, and in general such programs have been useful for reducing days open or increasing percentage of cows pregnant by three to five months postpartum. These programs require additional investments in drugs and veterinary costs and do not lower services per conception, but they do allow reproduction in the herd to be controlled in a more systematic way.

For nearly all estrous-synchronization systems, conception rate is better for cows inseminated on the basis of estrus that at a preselected time. The one exception to this is a system that was reported first in the summer of 1994 (16, 17). In this system, cows are given GnRH to induce emergence of a new wave of follicles (and in some cases ovulation), followed seven days later by prostaglandin to induce luteolysis, which is followed two days later by GnRH to induce ovulation and cows are inseminated 24 hours after this second GnRH, without regard to estrus. Early field trials of this system showed conception rates equal to those for cows inseminated at natural estrus and a 30 day reduction in days open among treated cows compared to their herdmates.

Passive Mount Detectors

Passive heat detection aids such as chalking, tail-painting and KaMaR Heatmount Detectors are beneficial for improving detection rate when combined with careful observation and good judgment (22). When such aids are used, accuracy of heat detection increases when more than one criterion is used to identify cows in estrus. When used alone, these aids may lead to a higher error rate and therefore no real improvement in detection rate over that of a casual system, unless their use is closely tied to programmed breeding where the time of estrus is highly predictable. For example, use of chalking or tail-painting in combination with prostaglandin or progestogens for synchronization of estrus may lead to detection of more animals in heat than would be the case with observation alone.

Heat Detector Animals

Treatment of heifers or cull cows with testosterone enhances their sexual activity to the point where they act like bulls in search of estrual cows (10, 14). When fitted with halter-mounted marking devices, these detector animals provide around-the-clock surveillance for cows in heat. They improve accuracy of detection of estrus and show more mounting activity than normal cows or heifers. Heifers can be induced to be more sexually aggressive by inserting four implants containing 200 mg testosterone propionate and 160 mg estradiol benzoate into each ear. It is recommended that the implants be replaced every 90 days.

Electronic Detection

Several electronic devices have been or are being developed for detection of estrus. This included devices for:

monitoring increased physical activity associated with estrus (electronic pedometers),
detection of mounts (rump-mounted or implantable devices),
detection of changes in electrical impedance in the vaginal submucosa (20) or vaginal mucus, and
detection of changes in vaginal temperature (18).

The devices that are fixed to or implanted in the cow also include circuitry for electronic identification. Such systems require personal computer systems for electronically identifying cows and "reading" signals from the devices. These systems allow one to automate monitoring of estrus-related activities and to reduce time required for observation. Nevertheless, the rate of estrous detection of such systems seldom exceeds that which can be achieved by careful, systematic observation of cattle. Nearly all of the devices that capture activity or mounting information utilize algorithms to interpret the data, and there is still a need to develop better methods for distinguishing between estrual and non-estrual cows (12).

In loose-housed cattle or those that are turned out of tie-stall barns for at least five to eight hours daily, activity monitors (pedometers) and mount detectors accurately identify about 70% of heats (20). Devices that measure electrical impedance of the vaginal submucosa or vaginal mucus do not require that cows are free to interact. At present, there are no commercially-available implantable devices that measure impedance, and the vaginal probes for measuring electrical conductivity are labour intensive.

Economics of Heat Detection


For comparisons of heat detection programs, a simple spreadsheet can be used to assess reproductive performance and economic returns from various heat detection systems (Figure 1). I have simulated the additional economic returns from using two organized heat detection programs compared to the typical program on most farms. The typical heat detection program is referred to as a Casual program, where cows are observed for heat when chores are being done, but there is no systematic effort to observe cows on a scheduled basis. The Systematic program involves regularly-scheduled observations (30 minutes twice daily) in addition to casual observations during chores. The Rigorous program involves regularly scheduled observations plus judicious use of heat detection aids (detector animal, tail painting, etc.) to supplement the systematic observations.

The parameters used for simulation of the various programs are shown in Table 5. Detection rates and accuracy of detection were based on values in the literature.

Fertility level of the herd was considered to be the innate fertility independent of errors in heat detection. Thus, herd fertility included effects due to herd health, heat stress, semen quality, skill of inseminators, production level, etc. It was varied from 30 to 90%, indicating that if detection was accurate 30 to 90% of the inseminations would result in conception. For this simulation, the voluntary waiting period was 60 days and cows were inseminated until 180 days postpartum. The economic data used in the simulations included costs per cow associated with more labor for heat detection (20 per minute) and $5 per cow for heat detection aids (for the Rigorous group).

The simulation indicated that returns to investments in heat detection programs were greatest in situations where herd fertility was lower (Table 6). The relative net returns per cow for Systematic and Rigorous heat detection programs decreased as herd fertility increased. This is because high fertility leads to relatively good reproductive performance even when submission rates and accuracy rates are low. The simulation indicates that over US$100 per cow in net returns could accrue from improved heat detection programs in herds with low to moderate fertility. Basically these returns arise because of more opportunities to get the cow bred within the designated time span and lower heat detection error rates that result in higher realized fertility.

References


1. Allrich, R.D. 1993. Estrous behavior and detection in cattle. pp. 249-262 in Vet. Clinics of North America: Food Animal Practice. W. F. Braun, Jr., R. S. Youngquest (eds.) W. B. Saunders Co., Philadelphia, PA.
2. Allrich, R.D. 1994. Endocrine and neural control of estrus in dairy cattle. J. Dairy Sci. 77: 2738-2744.
3. Britt, J.H. 1987. Detection of oestrus in cattle. pp 74-80 in The Veterinary Annual, C. S. G. Grunsell, F. W. G. Hill and M.-E. Raw (eds.), Scientechnica, Bristol, U.K.
4. Britt, J.H., R.G. Scott, J.D. Armstrong and M.D. Whitacre. 1986. Determinants of estrous behavior in lactating Holstein cows. J. Dairy Sci. 69:2195-2202.
5. Collick, D.W., W.R. Ward and H. Dobson. 1989. Associations between types of lameness and fertility. Vet. Rec. 125: 103-106.
6. Fonseca, F.A., J.H. Britt, B.T. McDaniel, J.C. Wilk and A.H. Rakes. 1983. Reproductive traits of Holsteins and Jerseys. Effects of age, milk yield and clinical abnormalities on involution of cervix and uterus, ovulation, estrous cycles, detection of estrus, conception rate and days open. J. Dairy Sci. 66:1128-1147.
7. Harrison, R.O., S.P. Ford, J.W. Young, A.J. Conley and A.E. Freeman. 1990. Increased milk production versus reproductive and energy status of high producing dairy cows. J. Dairy Sci. 73: 2749-2758.
8. Heersche, G., Jr. and R.L. Nebel. 1994. Measuring efficiency and accuracy of detection of estrus. J. Dairy Sci. 77: 2754-2761.
9. Helmer, S.D. and J.H. Britt. 1985. Mounting behavior as affected by stage of estrous cycle in Holstein heifers. J. Dairy Sci. 68:1290-1296.
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11. Kitwood, S.E., C.J.C. Phillips and M. Weise. 1993. Use of a vaginal mucus impedance meter to detect estrus in the cow. Theriogenology 40: 559-569.
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15. Nebel, R.L., W.D. Whittier, B.G. Cassell and J.H. Britt. 1987. Comparison of on-farm and laboratory milk progesterone assays for identifying errors in detection of estrus and diagnosis of pregnancy. J. Dairy Sci. 70: 1471-1476.
16. Pursley, J.R. , M.R. Kosorok and M.C. Wiltbank. 1994b. Reproductive management of lactating dairy cows using synchronization of ovulation. J. Dairy Sci. 77 (Suppl. 1) 69.
17. Pursley, J.R., M.O. Mee, M.D. Brown and M.C. Wiltbank. 1994a. Synchronization of ovulation in dairy cattle using GnRH and PGF2a. J. Dairy Sci. 77 (Suppl. 1): 230.
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19. Reimers, T.J., R.D. Smith, and S.K. Newman. 1985. Management factors affecting reproductive performance of dairy cows in the northeastern United States. J. Dairy Sci. 68: 963.
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21. Spicer, L.J., W.B. Tucker and G.D. Adams. 1990. Insulin-like growth factor-I in dairy cows: Relationships among energy balance, body condition, ovarian activity, and estrous behavior. J. Dairy Sci. 73: 929.
22. Stevenson, J.S. and J.H. Britt. 1977. Detection of estrus by three methods. J. Dairy Sci. 60:1994-1998.
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25. Vailes, L.D., S.P. Washburn and J.H. Britt. 1992. Effects of various steroid milieus or physiological states on sexual behavior of Holstein cows. J. Anim. Sci. 70: 2094-2103.

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